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Journal of Nutritional Science

According to The Menopause Society, the benefits of hormone therapy generally outweigh the risks for many healthy women who are under age 60 or within 10 years of menopause

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Apoptosis, another consequence of oxidative damage and mitochondrial dysfunction, was evaluated using Annexin-V staining, which detects early apoptotic changes by binding to phosphatidylserine exposed on the outer leaflet of the plasma membrane

The blocking solution was removed and 150 L primary antibody (711,500, Rabbit Occludin Polyclonal Antibody, ThermoFisher Scientific) diluted in blocking solution (8.33 g/mL) was added